Buy 4-HO-MET 20 mg pellets Online
4-Ho-Met 20 mg Pellets, 4-HO-MET is short for 4-Hydroxy-N-methyl-N-ethyltryptamine. This substance falls into the tryptamine class of chemicals. It is very similar to psilocin, which is the primary psychoactive component in magic mushrooms. 4-HO-MET is able to generate psychedelic results by acting on the serotonin receptors. Many researchers have noted that experimental findings for 4-HO-MET are nearly identical to those of psilocin. It is common for 4-HO-MET experiments to yield results of color enhancement, drifting, pattern recognition enhancement, symmetrical texture repetition, transformations, internal hallucinations, conceptual thinking, and analysis enhancement. A sense of ego death is another common finding with 4-HO-MET experiments, keep in mind that it is an analogue of 4-AcO-MET.
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The pellet is resuspended in 20 μg/ml PI in PBS containing 1 mg/ml of RNase A and the cells are transferred to polystrene 12 × 75 mm Falcon tubes. Generally, 1–2 ml of the PI stain is adequate for 106 cells. The PI is prepared from a 100× stock (2 mg/ml) but the RNase is added fresh each time. The tubes are placed in the dark at room temperature for 2 h prior to FACS analysis. They may also be stored overnight at 4°C in the dark. The cells are treated with doxorubicin. The stock (0.1 mg/ml) is thawed and added directly to cell cultures. A dose–response curve for each cell line should be performed in order to attain a dose that produces a p53-dependent response. This can be done as a single parameter experiment using PI before setting up a BrdU incorporation assay.